Enhanced aptasensor performance for targeted HER2 breast cancer detection by using screen-printed electrodes modified...

Area of Science:

• Biosensor development
• Electrochemical detection
• Nanomaterials in biosensing

Background:

• Human epidermal growth factor receptor 2 (HER2) is a key biomarker in certain cancers.
• Accurate and sensitive detection of HER2 is crucial for diagnosis and treatment monitoring.
• Existing detection methods may lack sensitivity, specificity, or speed.

Purpose of the Study:

• To develop a novel aptamer-based biosensor for selective HER2 detection.
• To achieve high sensitivity and specificity using a screen-printed electrode platform.
• To evaluate the performance characteristics of the developed aptasensor.

Main Methods:

• Immobilization of HER2 aptamers on gold nanoparticle-modified screen-printed carbon electrodes via electrostatic adsorption.
• Electrochemical characterization using Cyclic Voltammetry (CV), Differential Pulse Voltammetry (DPV), and Electrochemical Impedance Spectroscopy (EIS).
• Surface analysis using Fourier Transform Infrared Spectroscopy (FTIR) and Atomic Force Microscopy (AFM).

Main Results:

• The aptasensor demonstrated a rapid response with a 5-minute binding time.
• A wide linear dynamic range from 0.001-100 ng/mL was observed.
• High sensitivity (52.85 μA/ng/mL), low detection limit (0.001 ng/mL), and excellent selectivity with minimal interference (~8%) were achieved.

Conclusions:

• The developed aptasensor offers a sensitive, specific, and rapid method for HER2 detection.
• The use of gold nanoparticles enhances aptamer immobilization and sensor performance.
• This aptasensor holds potential for clinical applications in HER2 diagnostics.