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Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
Published on: April 7, 2017
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LINC00662 facilitates osteosarcoma progression via sponging miR-103a-3p and regulating SIK2 expression
Jianghu Huang1,2, Feiyue Lin1,2, Chuncai Xu1,2
1Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian, China.
Journal of Tissue Engineering and Regenerative Medicine
|September 24, 2021
Summary
Long intergenic non-protein coding RNA 662 (LINC00662) promotes osteosarcoma (OS) progression. LINC00662 facilitates OS cell malignant behaviors and tumor growth via the miR-103a-3p/SIK2 pathway.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long non-coding RNAs (lncRNAs) are implicated in cancer regulation.
- Long intergenic non-protein coding RNA 662 (LINC00662) function in osteosarcoma (OS) remains unclear.
Purpose of the Study:
- To investigate the role and mechanism of LINC00662 in osteosarcoma.
- To elucidate the LINC00662/microRNA/gene axis in OS progression.
Main Methods:
- Quantitative real-time PCR to assess gene expression.
- Cell proliferation, migration, and invasion assays.
- Bioinformatic analysis and dual-luciferase reporter assays to confirm interactions.
Main Results:
- LINC00662 expression is upregulated in OS cells.
- LINC00662 downregulation inhibits OS cell malignant behaviors and tumor growth.
- LINC00662 acts as a sponge for miR-103a-3p, and SIK2 is a downstream target, forming the LINC00662/miR-103a-3p/SIK2 axis that promotes OS.
Conclusions:
- LINC00662 promotes osteosarcoma progression by regulating the miR-103a-3p/SIK2 axis.
- LINC00662 may serve as a potential therapeutic target for osteosarcoma treatment.
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