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Engineering a 3D Vascularized Adipose Tissue Construct Using a Decellularized Lung Matrix.

Megan K DeBari1, Wai Hoe Ng2, Mallory D Griffin2

  • 1Department of Materials Science & Engineering, Carnegie Mellon University, Pittsburgh, PA 15203, USA.

Biomimetics (Basel, Switzerland)
|September 25, 2021
PubMed
Summary
This summary is machine-generated.

This study engineered perfusable, pre-vascularized adipose tissue grafts using a decellularized lung matrix (DLM) and patient cells. This innovative approach addresses challenges in reconstructing large adipose tissue defects, improving graft survival and function.

Keywords:
adipocytesadiposedecellularized extracellular matrixdefect fillingendothelial cellsperfusion culturestem cellssubcutaneoustissue engineeringvascularized graft

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Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Tissue Engineering

Background:

  • Critically sized subcutaneous white adipose tissue defects cause disfigurement and dysfunction, posing a reconstructive challenge.
  • Graft failure in large defects is often due to insufficient vascularization.
  • Existing methods lack effective solutions for large-scale adipose tissue reconstruction.

Purpose of the Study:

  • To develop a method for engineering perfusable, pre-vascularized, high-density adipose grafts.
  • To utilize a decellularized lung matrix (DLM) combined with patient-derived adipose cells.
  • To assess the viability and integration of adipose cells and endothelial cells within the DLM.

Main Methods:

  • Harvesting and decellularizing lung tissue to create a decellularized lung matrix (DLM).
  • Seeding the DLM with patient-derived adipose cells (adipocytes) and human adipose-derived stem cells (hASCs).
  • Culturing the engineered grafts under static and perfusion conditions, and assessing cell integration with endothelial cells.

Main Results:

  • Successful delivery and sustained presence of adipocytes and hASCs within the DLM's alveolar space.
  • Adipose-derived cells maintained morphology and function in DLM cultures, with enhanced outcomes under perfusion.
  • Endothelial cells integrated into the DLM's vascular network alongside adipocytes.

Conclusions:

  • The decellularized lung matrix (DLM) is a viable platform for creating vascularized adipose tissue grafts.
  • Engineered adipose grafts demonstrate potential for addressing large subcutaneous adipose tissue defects.
  • Perfusion culture enhances the functionality of adipose tissue engineered within the DLM.