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Immunosensor for Assessing the Welfare of Trainee Guide Dogs.

Hannah Perkins^1,2, Michelle Higgins^1,2, Marinara Marcato¹

¹Tyndall National Institute, University College Cork, T12 R5CP Cork, Ireland.

|September 25, 2021

Summary

This summary is machine-generated.

A new immunosensor accurately detects cortisol, the stress hormone, in canine saliva. This tool helps assess a dog's stress levels during training, aiding in suitability for roles like assistance dogs.

Keywords:

cortisol dogs graphene impedance sensor stress

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Area of Science:

Veterinary Medicine
Biomarker Development
Analytical Chemistry

Background:

Cortisol is a key biomarker hormone, commonly known as the stress hormone.
Measuring cortisol levels in dogs can indicate their stress response during training.
This assessment helps determine a dog's suitability for specific roles, such as assistance or companion dogs.

Purpose of the Study:

To develop and validate an immunosensor for detecting cortisol in canine saliva.
To assess the potential of this immunosensor in evaluating stress levels in dogs undergoing training.

Main Methods:

Development of an immunosensor utilizing electrochemical impedance spectroscopy (EIS).
Characterization of the sensor using chemical and topographical methods.
Calibration of the sensor across a cortisol concentration range of 0.0005 to 50 μg/mL.

Main Results:

The immunosensor demonstrated a theoretical limit of detection of 3.57 fg/mL.
Cortisol was successfully detected and quantified in canine saliva samples.
Measurements fell within relevant physiological ranges for dogs.

Conclusions:

The developed immunosensor is effective for measuring cortisol in canine saliva.
This technology can serve as a valuable tool for assessing stress in training dogs.
It aids in determining a dog's suitability for demanding roles.