Related Experiment Video
Updated: Oct 19, 2025

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
An Automated Coupled-Column Liquid Chromatography System for Measuring Aqueous Solubilities of Hydrophobic Solutes
John W Owens1, Howard DeVoe1, Thomas J Buckley2
1University of Maryland, College Park, MD 20742.
Abstract:
An automated apparatus is described for measuring the aqueous solubility of a sparingly soluble organic compound at many different temperatures. Water is pumped through a generator column packed with a chromatographic support coated with the organic compound, producing a saturated solution. The solute in a measured volume of this solution is extracted with an extractor column and analyzed by high performance liquid chromatography (HPLC). The temperature of the thermostat bath and the operation of the valves and the HPLC are under the control of a microcomputer. Solubility measurements of ethylbenzene obtained with this apparatus have a standard deviation at any one temperature of about 3% of the mean.
Related Concept Videos
High-Performance Liquid Chromatography: Instrumentation
High-Performance Liquid Chromatography: Elution Process
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Types of Detectors
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...
Analyte Adsorption and Distribution

