Related Experiment Video
Updated: Oct 19, 2025

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
Generator Columns and High Pressure Liquid Chromatography for Determining Aqueous Solubilities and Octanol-Water
Howard DeVoe1, Michele M Miller1, Stanley P Wasik1
1National Bureau of Standards, Washington, DC 20234.
Abstract:
Generator columns packed with a solid support and loaded with a liquid organic phase make it possible to rapidly and conveniently equilibrate water with the organic phase. By coupling the generator column to an extractor column for high pressure liquid chromatographic analysis of the aqueous solution, errors from surface adsorption and loss to the atmosphere are avoided. Using this method, the mean values and confidence limits at a 95 percent confidence level of the aqueous solubility, S, and the octanol-water partition coefficient, P, of n-propylbenzene at 25 °C were found to be S = (4.32 ± 0.02) × 10-4 M and log P = 3.720 ± 0.003.
More Related Videos
09:32A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy
Published on: January 30, 2019
11:44Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry
Published on: March 6, 2016
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
High-Performance Liquid Chromatography: Instrumentation
Principles Of Column Chromatography
High-Performance Liquid Chromatography: Elution Process
Types Of Column Chromatography
Gel Filtration Chromatography
When the...