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Related Experiment Video

Updated: Oct 19, 2025

Equibiaxial Stretching Device for High Magnification Live-Cell Confocal Fluorescence Microscopy
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Equibiaxial Stretching Device for High Magnification Live-Cell Confocal Fluorescence Microscopy

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On-chip integrated optical stretching and electrorotation enabling single-cell biophysical analysis.

Liang Huang1,2, Fei Liang1, Yongxiang Feng1

  • 1Department of Precision Instrument, State Key Laboratory of Precision Measurement Technology and Instrument, Tsinghua University, Beijing, China.

Microsystems & Nanoengineering
|September 27, 2021
PubMed
Summary

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This study introduces a novel microfluidic chip for measuring single-cell mechanical and electrical properties. The device uses optical fibers and electrodes to characterize cell biophysics, aiding cancer research.

Area of Science:

  • Biophysics
  • Cell Biology
  • Microfluidics

Background:

  • Cells possess unique intrinsic mechanical and electrical properties.
  • Characterizing these properties is crucial for understanding cell function and disease.
  • Existing methods may lack the multiparameter capabilities for comprehensive single-cell analysis.

Purpose of the Study:

  • To develop and validate a microfluidic chip for multiphysical parameter measurement of single cells.
  • To extract key mechanical and dielectric properties from individual cells.
  • To demonstrate the chip's utility in cancer research, specifically in studying epithelial-mesenchymal transition.

Main Methods:

  • A microfluidic chip integrating two opposing optical fibers for cell stretching and four 3D electrodes for cell rotation.
Keywords:
EngineeringPhysics

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Last Updated: Oct 19, 2025

Equibiaxial Stretching Device for High Magnification Live-Cell Confocal Fluorescence Microscopy
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  • Optical fibers capture and deform single cells; 3D electrodes induce cell rotation.
  • Analysis of stretching deformation and rotation spectra to determine biophysical parameters.

    • Main Results:

    • Successfully measured five biophysical parameters: shear modulus, steady-state viscosity, relaxation time, area-specific membrane capacitance, and cytoplasm conductivity.
    • Validated the chip's performance across five cell lines (HeLa, A549, HepaRG, MCF7, MCF10A).
    • Observed distinct cellular property changes in A549 cells undergoing TGF-β1-induced epithelial-mesenchymal transition (EMT).

    Conclusions:

    • The developed microfluidic chip enables simultaneous multiparameter characterization of single cells.
    • This platform offers a powerful tool for quantitative biophysical analysis of cells.
    • The chip has significant potential for advancing single-cell research, particularly in oncology and disease mechanism studies.