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Updated: Oct 18, 2025

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Current Scenario and Challenges in the Direct Identification of Microorganisms Using MALDI TOF MS
Sang-Soo Han1, Young-Su Jeong2, Sun-Kyung Choi2
1Advanced Defense Science & Technology Research Institute, Agency for Defense Development, Daejeon 34186, Korea.
Abstract:
MALDI TOF MS-based microbial identification significantly lowers the operational costs because of minimal requirements of substrates and reagents for extraction. Therefore, it has been widely used in varied applications such as clinical, food, military, and ecological research. However, the MALDI TOF MS method is laced with many challenges including its limitation of the reference spectrum. This review briefly introduces the background of MALDI TOF MS technology, including sample preparation and workflow. We have primarily discussed the application of MALDI TOF MS in the identification of microorganisms. Furthermore, we have discussed the current trends for bioaerosol detection using MALDI TOF MS and the limitations and challenges involved, and finally the approaches to overcome these challenges.
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