Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Compost and rootstock effects on fibrous root physiology and bacterial community composition in young citrus trees under endemic Huanglongbing.

Scientific reports·2026

Same author

Cryptic Evolution of Heteroresistance as Adaptation to Treatment Interruptions.

bioRxiv : the preprint server for biology·2026

Same author

Early Spatiotemporal Deficits Become Competitive Advantages During Collective Expansion.

bioRxiv : the preprint server for biology·2026

Same author

High-resolution, high-throughput detection of hidden antibiotic resistance with the dilution-and-delay (DnD) susceptibility assay.

Nature communications·2026

Same author

Mapping single-cell responses to population-level dynamics during antibiotic treatment.

Molecular systems biology·2026

Same author

Efflux pumps control intracellular drug-target kinetics by limiting rebinding in bacteria.

Science advances·2026

Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 18, 2025

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli

Published on: March 24, 2023

Observing Bacterial Persistence at Single-Cell Resolution.

Emma Dawson¹, Emrah Şimşek^1,2, Minsu Kim^3,4

¹Department of Physics, Emory University, Atlanta, GA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|September 30, 2021

Summary

This summary is machine-generated.

Researchers developed a new microscopy method to observe antibiotic-tolerant bacterial persisters. This technique allows for long-term, stable single-cell imaging, aiding in the study of bacterial persistence and lag phases.

Keywords:

Antibiotic resistance Lag time Persistence Persisters Single-cell microscopy

More Related Videos

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Published on: February 14, 2025

Single-cell Microfluidic Analysis of Bacillus subtilis

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

Related Experiment Videos

Last Updated: Oct 18, 2025

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli

Published on: March 24, 2023

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Published on: February 14, 2025

Single-cell Microfluidic Analysis of Bacillus subtilis

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

Area of Science:

Microbiology
Cell Biology
Microscopy Techniques

Background:

Bacterial populations contain persister cells, a subpopulation exhibiting antibiotic tolerance.
Persister cells are characterized by a prolonged lag phase, making them difficult to study.
Long-term single-cell observation is crucial for understanding bacterial persistence.

Purpose of the Study:

To describe a novel method for long-term single-cell microscopy observation.
To apply this method for characterizing the lag phase and persistence of individual bacterial cells.
To enable high-quality quantitative data acquisition on bacterial persistence.

Main Methods:

Development of a single-cell imaging method utilizing glass-bottom dishes.
Incorporation of a nutrient agarose pad to maintain a stable cellular environment.
Application of long-term microscopy for observing bacterial lag phase and persistence.

Main Results:

The described method facilitates stable, long-term observation of individual bacterial cells.
Successfully applied the technique to characterize lag phase duration in Escherichia coli.
Enabled quantitative analysis of bacterial persistence at the single-cell level.

Conclusions:

The developed microscopy technique is effective for studying bacterial persister phenotypes.
This method overcomes limitations of previous approaches requiring extended observation periods.
Provides a valuable tool for advancing the understanding of antibiotic tolerance and bacterial survival strategies.