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Related Experiment Video

Updated: Oct 18, 2025

Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility
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Hydrogel Formation with Enzyme-Responsive Cyclic Peptides.

Andrea S Carlini1,2,3,4,5,6, Mary F Cassidy1,2,3,4,5,6, Nathan C Gianneschi7,8,9,10,11,12

  • 1Department of Chemistry, Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 1, 2021
PubMed
Summary
This summary is machine-generated.

Injectable peptide hydrogels are engineered for minimally invasive delivery. These enzyme-responsive materials prevent clogging and enable controlled self-assembly at the target site.

Keywords:
Enzyme-responsiveHydrogelsInjectableMacrocyclesMinimally invasiveProteolytic cleavageSelf-assembling peptidesSteric constraintTissue engineering

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Area of Science:

  • Biomaterials Science
  • Chemical Engineering
  • Nanotechnology

Background:

  • Self-assembling peptides (SAPs) form hydrogels for tissue engineering and drug delivery.
  • Injectable SAPs require shear-thinning and post-injection reassembly, which is challenging.
  • Current SAPs often clog needles or fail to reassemble.

Purpose of the Study:

  • To provide a protocol for topological control of enzyme-responsive peptide-based hydrogels.
  • To develop injectable hydrogels with shear-thinning and reassembly capabilities.
  • To overcome limitations of current self-assembling peptides for minimally invasive delivery.

Main Methods:

  • Formulating sterically constrained cyclic peptide progelators.
  • Utilizing enzyme-responsive linearization for hydrogelation.
  • Demonstrating flow through catheters and post-injection gelation.

Main Results:

  • Developed injectable peptide progelators that disrupt self-assembly during injection.
  • Achieved successful flow through catheters without clogging.
  • Induced hydrogelation upon exposure to target enzymes at the desired site.

Conclusions:

  • Topological control of enzyme-responsive peptide hydrogels enables successful minimally invasive delivery.
  • This approach overcomes clogging and reassembly issues associated with injectable SAPs.
  • The developed materials show promise for advanced tissue engineering and drug delivery applications.