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Depth resolution in multifocus laser speckle contrast imaging.

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    Laser speckle contrast imaging (LSCI) accuracy is improved by correcting focus-dependent blur. This new method provides depth-resolved blood flow measurements in vivo.

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    Area of Science:

    • Biomedical Optics
    • Medical Imaging
    • Physiology

    Background:

    • Laser speckle contrast imaging (LSCI) assesses blood flow using speckle statistics.
    • Out-of-focus blur significantly degrades LSCI accuracy.
    • Accurate blood flow quantification is crucial for understanding physiological processes.

    Purpose of the Study:

    • To develop and validate a deconvolution strategy for correcting out-of-focus blur in LSCI.
    • To demonstrate depth-resolved LSCI for simultaneous multi-plane imaging.
    • To improve the accuracy of blood flow speed estimation in biological tissues.

    Main Methods:

    • A z-splitter was employed for instantaneous multifocus imaging.
    • A deconvolution strategy was implemented to correct for focus-dependent blur.
    • Depth-resolved LSCI was applied to in vivo imaging of mouse cortex vasculature.

    Main Results:

    • The fraction of dynamic versus static light scattering was shown to be focus-dependent.
    • The deconvolution strategy effectively corrected for out-of-focus blur.
    • Depth-resolved LSCI provided simultaneous structural and flow-speed information.
    • Improved blood flow speed estimates were achieved across a 300µm depth range.

    Conclusions:

    • Focus-dependent blur can be corrected using a deconvolution strategy.
    • Depth-resolved LSCI enables robust, multi-plane imaging of blood flow.
    • This technique enhances the accuracy of in vivo blood flow measurements.