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Published on: September 10, 2016
Quinoa sourdough-based biscuits with high antioxidant activity fermented with autochthonous lactic acid bacteria
Sergio H Sandez Penidez1, Marina A Velasco Manini1, Jean Guy LeBlanc1
1Centro de Referencia para Lactobacilos (CERELA)-CONICET, San Miguel de Tucumán, Argentina.
Aims:
To evaluate the capacity of autochthonous lactic acid bacteria (LAB) (43) from Andean grains to increase the antioxidant activity (AOA) and total phenolic compounds (TPCs) in quinoa sourdough to select best performing strains to be used as starter cultures in the elaboration of biscuits.
Methods And Results:
Microbial growth (CFU per g) and pH were evaluated during quinoa dough fermentation. Counts were increased in a range of 0.61-2.97 log CFU per g and pH values between 3.95 and 4.54 were determined after 24 h at 30°C of fermentation. Methanolic (ME) and aqueous (AE) extracts were obtained at the end of fermentation, and free radical scavenging capacity was performed by the DPPH and ABTS methods. ME was selected for further analysis using other methods and TPC quantification. Principal component analysis showed the highest scores of growth, acidification capacity, AOA and TPC for the strains Lc. mesenteroides subsp. mesenteroides CRL 2131 and L. plantarum CRL 1964 and CRL 1973. AOA and TPC in biscuits made with sourdough from these LAB were higher than the acidified and uninoculated controls.
Conclusions:
Autochthonous LAB strains (3) increased the AOA of quinoa-based biscuits.
Significance And Impact Of The Study:
Quinoa sourdough obtained with selected LAB is suitable as an ingredient for bakery foods with improved antioxidant status.
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