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Multiple approaches to oral epithelial dysplasia degree analyses: a pilot study
Carla M Ramírez-Martínez1, David A Trejo-Remigio2, Emiliano Jurado-Castañeda3
1School of Dentistry, Postgraduate and Research Division, Department of Oral Medicine and Pathology, National Autonomous University of Mexico, Mexico City, Mexico.
Minerva Dental and Oral Science
|October 5, 2021
Summary
Software-assisted analysis correlates with oral epithelial dysplasia (OED) severity. MMP1 is a key marker, and higher Bax expression suggests a potential therapeutic target for OED progression.
Area of Science:
- Oral pathology
- Molecular biology
- Cancer research
Background:
- Oral epithelial dysplasia (OED) represents precancerous changes in oral tissues.
- Understanding molecular markers associated with OED severity is crucial for early detection and intervention.
Purpose of the Study:
- To investigate the relationship between molecular marker expression and histological features of oral dysplasia.
- To evaluate software-assisted nuclear morphology measurement as a tool for OED grading.
Main Methods:
- Analysis of 15 OED samples across severity grades using software-assisted image cytometry.
- Immunohistochemical determination of p53, Bax, and Bcl2 expression.
- RT-PCR for MMP1, MMP2, MMP9, and hTERT gene expression.
Main Results:
- Software-assisted nuclear morphology measurements showed congruence with OED severity.
- MMP1 was the predominantly expressed matrix metalloproteinase, followed by MMP9 and MMP2.
- hTERT expression significantly correlated with OED severity; Bax expression exceeded Bcl-2 expression.
Conclusions:
- Software-assisted measurement is a viable alternative for determining OED severity.
- MMP1 is a significant marker for OED severity.
- The pro-apoptotic state indicated by higher Bax expression may offer a therapeutic target to mitigate OED progression.
