Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

17.4K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
17.4K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

9.1K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
9.1K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.6K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hyperspectral tomographic diffractive microscopy: Development and applications.

Journal of microscopy·2026
Same author

Dual-view tomographic diffraction microscopy.

Optics express·2025
Same author

Evaluation of micro-hologram activation for dynamic display applications.

Optics express·2024
Same author

Roadmap on Label-Free Super-Resolution Imaging.

Laser & photonics reviews·2024
Same author

Recent Advances and Current Trends in Transmission Tomographic Diffraction Microscopy.

Sensors (Basel, Switzerland)·2024
Same author

Jones tomographic diffractive microscopy with a polarized array sensor.

Optics express·2023
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Oct 17, 2025

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

11.7K

Optimizing sample illumination scanning for reflection and 4Pi tomographic diffractive microscopy.

Asemare Mengistie Taddese, Nicolas Verrier, Matthieu Debailleul

    Applied Optics
    |October 6, 2021
    PubMed
    Summary
    This summary is machine-generated.

    Optimal scanning in tomographic diffractive microscopy (TDM) is crucial for high-resolution, label-free imaging. This study found that 3D uniform scanning best fills Fourier space, improving image quality and refractive index estimation in reflection and 4Pi TDM systems.

    More Related Videos

    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
    08:53

    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

    Published on: August 15, 2014

    9.9K
    Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
    08:46

    Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

    Published on: April 13, 2016

    10.2K

    Related Experiment Videos

    Last Updated: Oct 17, 2025

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    11.7K
    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
    08:53

    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

    Published on: August 15, 2014

    9.9K
    Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
    08:46

    Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

    Published on: April 13, 2016

    10.2K

    Area of Science:

    • Optics and Photonics
    • Microscopy Techniques
    • Biomedical Imaging

    Background:

    • Tomographic diffractive microscopy (TDM) offers high-resolution, label-free imaging.
    • Fast TDM acquisitions require minimizing recorded holograms.
    • Image reconstruction quality depends on optimal Fourier space filling via illumination scanning.

    Purpose of the Study:

    • To theoretically investigate reflection TDM and 4Pi TDM.
    • To determine optimal angular sweeping strategies for TDM.
    • To enhance image quality and refractive index estimation in TDM.

    Main Methods:

    • Theoretical study of reflection TDM and 4Pi TDM configurations.
    • Image simulations to evaluate different angular sweeping patterns.
    • Analysis of Fourier space filling efficiency for various scanning methods.

    Main Results:

    • Three-dimensional uniform scanning was identified as the optimal strategy.
    • This method provides superior Fourier space filling for both reflection and 4Pi TDM.
    • Improved refractive index estimation accuracy was achieved.

    Conclusions:

    • 3D uniform scanning is the most effective illumination strategy for reflection and 4Pi TDM.
    • This approach enhances image reconstruction and quantitative phase measurements.
    • The findings contribute to optimizing TDM for advanced imaging applications.