Related Experiment Video
Updated: Oct 17, 2025

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Structure and gene cluster of the O-antigen of Enterobacter cloacae G3422
Andrei V Perepelov1, Andrei V Filatov1, Wenxuan Zhu2
1N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
Abstract:
The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G3422 and studied by chemical methods, including sugar analyses, Smith degradation, and solvolysis with anhydrous trifluoroacetic acid, along with 1H and 13C NMR spectroscopy. The following structure of the branched tetrasaccharide repeating unit was established: The O-antigen gene cluster of Enterobacter cloacae G3422 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in a good agreement with the O-antigen structure.
Related Concept Videos
Prokaryotic Gene Structure and Organization
Gram-negative Bacterial Protein Secretion Systems
Formation of Lipopolysaccharides
Operons
Repressible Operon: trp Operon
Operon Model

