Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

RepC is rate limiting for pT181 plasmid replication.

J N Manch-Citron, M L Gennaro, S Majumder

    Plasmid
    |September 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Building a Synthetic Cell Together.

    Nature communications·2025
    Same author

    An all phosphorene lattice nanometric spin valve.

    Scientific reports·2024
    Same author

    Comparative Efficacy and Safety of Amlodipine and Losartan Potassium in Essential Hypertension in a Tertiary Hospital of Bangladesh.

    Mymensingh medical journal : MMJ·2024
    Same author

    Quench dynamics of edge states in a finite extended Su-Schrieffer-Heeger system.

    Physical review. E·2023
    Same author

    Identification and classification of arrhythmic heartbeats from electrocardiogram signals using feature induced optimal extreme gradient boosting algorithm.

    Computer methods in biomechanics and biomedical engineering·2023
    Same author

    Impact of DHCWs' Safety Perception on Vaccine Acceptance and Adoption of Risk Mitigation Strategies.

    JDR clinical and translational research·2022
    Same journal

    Enhanced resistance to macrolides and tetracyclines due to cooperation among plasmid-encoded genes associated with IS26 mobile genetic elements.

    Plasmid·2026
    Same journal

    iBiT: A vector system allowing stable genome integration and scalable expression of NanoLuc fusion proteins to monitor dynamic protein-protein interactions in intact cells.

    Plasmid·2026
    Same journal

    Comparative genomics of diverse Escherichia coli O157:H7 strains to characterize plasmids, prophages, virulence and antimicrobial resistance genes.

    Plasmid·2025
    Same journal

    Megaplasmids of the enteropathogenic species Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus represent a group of novel genetic elements unrelated to other plasmids of Vibrionaceae.

    Plasmid·2025
    Same journal

    Assembly-based analysis of the infant gut microbiome reveals novel ubiquitous plasmids.

    Plasmid·2025
    Same journal

    Expression, purification, and refolding of an optimized SARS-CoV-2 receptor binding domain in E. coli.

    Plasmid·2025
    See all related articles

    Replication of the pT181 plasmid is controlled by the RepC initiator protein concentration. Studies show RepC synthesis regulates pT181 plasmid replication, with a secondary regulatory mechanism yet to be identified.

    Area of Science:

    • Molecular Biology
    • Plasmid Biology
    • Bacterial Genetics

    Background:

    • The pT181 plasmid is a well-characterized extrachromosomal element in bacteria.
    • Understanding plasmid replication mechanisms is crucial for genetic engineering and biotechnology.
    • The RepC protein is known to be essential for pT181 replication initiation.

    Purpose of the Study:

    • To investigate the role of RepC protein concentration in regulating pT181 plasmid replication.
    • To determine if RepC synthesis is the rate-limiting step for pT181 replication.
    • To explore the existence of secondary regulatory mechanisms governing pT181 copy number.

    Main Methods:

    • Analysis of pT181 plasmid replication rates under varying RepC concentrations.
    • Use of chloramphenicol (Cm) to inhibit protein synthesis and observe replication turnoff.

    Related Experiment Videos

  • Construction of a translational fusion between the repC gene and a Cm-inducible reporter gene (cat-86).
  • Monitoring plasmid copy number amplification upon Cm induction.

    • Main Results:

    • Plasmid replication ceased immediately upon addition of inhibitory chloramphenicol concentrations, unlike chromosomal replication.
    • A translational fusion construct led to an eightfold amplification of both the fusion plasmid and a co-resident pT181 plasmid.
    • Amplified plasmids reached stable, elevated copy numbers, not uncontrolled runaway replication.
    • These findings indicate RepC is rate-limiting and suggest a secondary regulatory control.

    Conclusions:

    • RepC protein synthesis is the primary determinant of pT181 plasmid replication rate.
    • pT181 plasmid replication is regulated at the level of RepC synthesis.
    • A secondary, yet unidentified, regulatory mechanism maintains stable elevated plasmid copy numbers.