Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation.

Nature methods·2023

Same author

STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity.

Nature communications·2022

Same author

Mapping molecular complexes with super-resolution microscopy and single-particle analysis.

Open biology·2022

Same author

New insights into promoter-enhancer communication mechanisms revealed by dynamic single-molecule imaging.

Biochemical Society transactions·2021

Same author

An evaluation of the Unyvero pneumonia system for rapid detection of microorganisms and resistance markers of lower respiratory infections-a multicenter prospective study on ICU patients.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2021

Same author

Nucleic Acid-based Testing for Noninfluenza Viral Pathogens in Adults with Suspected Community-acquired Pneumonia. An Official American Thoracic Society Clinical Practice Guideline.

American journal of respiratory and critical care medicine·2021

Same journal

Author Correction: Improved RNA base editing with guide RNAs mimicking highly edited endogenous ADAR substrates.

Nature biotechnology·2026

Same journal

Unlocking the chemical potential of filamentous fungi using prime editing.

Nature biotechnology·2026

Same journal

A genome-scale CRISPRi perturbation atlas of human induced pluripotent stem cells.

Nature biotechnology·2026

Same journal

Prime editing for precise genome engineering and modulation of fungal metabolism.

Nature biotechnology·2026

Same journal

Retargeted serine integrases for one-step, precise integration of large DNA sequences in human cells.

Nature biotechnology·2026

Same journal

A retargeted recombinase for precise insertion of large DNA.

Nature biotechnology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 17, 2025

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Published on: January 30, 2020

Volumetric interferometric lattice light-sheet imaging.

Bin Cao¹, Simao Coelho¹, Jieru Li¹

¹Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.

Nature Biotechnology

|October 12, 2021

Summary

This summary is machine-generated.

Researchers developed 3D interferometric lattice light-sheet (3D-iLLS) imaging for high-resolution live cell analysis. This technique improves spatiotemporal resolution and detection sensitivity for studying cellular dynamics.

More Related Videos

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Published on: March 6, 2013

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

Related Experiment Videos

Last Updated: Oct 17, 2025

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Published on: January 30, 2020

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Published on: March 6, 2013

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

Area of Science:

Cellular biology
Microscopy techniques
Biophysics

Background:

Live cell imaging is crucial for understanding cellular dynamics.
Current methods face limitations in speed, excitation intensity, and background noise, hindering high-resolution 4D data acquisition.
Extracting detailed spatiotemporal information from live cells remains a significant challenge.

Purpose of the Study:

To introduce a novel imaging technique, 3D interferometric lattice light-sheet (3D-iLLS), for enhanced live cell imaging.
To overcome the limitations of existing methods by improving resolution, sensitivity, and background suppression.
To enable detailed analysis of subcellular structures and dynamics in 4D.

Main Methods:

Development of 3D interferometric lattice light-sheet (3D-iLLS) imaging.
Integration of 4Pi interferometry with selective plane illumination.
Application of the technique to various biological systems including mRNA, transcription regulators, cytoskeleton, and mitochondria.

Main Results:

Achieved an axial resolution of 100 nm (FWHM) and single-particle localization precision of <10 nm (1σ).
Demonstrated high background suppression and improved volumetric resolution.
Successfully imaged diverse subcellular components with enhanced clarity and detail.

Conclusions:

3D-iLLS imaging offers a significant advancement in live cell microscopy.
The technique provides unprecedented 4D resolution and signal-to-noise ratio for subcellular analysis.
This method will accelerate the study of complex biological processes at the nanoscale.