Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of blood contamination on hydrophilic metabolomics in human meningioma tissue.

Analytical and bioanalytical chemistry·2026
Same author

A Barcoding Strategy for Pooled Single-Cell LA-ICP-TOFMS Analysis of Metal-Containing Therapeutics.

Analytical chemistry·2026
Same author

A multi-dimensional approach combining HPLC, ion mobility and high resolution mass spectrometry for investigating transition metal complexes.

Scientific reports·2026
Same author

Rapid quantitative bioanalysis using LC-HRIM-QTOFMS with flexible sample delivery and variable traveling wave profiles.

Analytica chimica acta·2026
Same author

Characterization of black oat root exudates in the presence of interspecific weed species and intraspecific neighbors, and their effects on root traits.

Frontiers in plant science·2026
Same author

Evaluating limits of detection for single-cell elemental bioimaging by LA-ICP-TOFMS.

Talanta·2026

Related Experiment Video

Updated: Oct 17, 2025

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
07:25

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: January 5, 2021

4.6K

Yeast-based reference materials for quantitative metabolomics.

Hendri Wasito1,2, Gerrit Hermann3,4, Veronika Fitz5

  • 1Institute of Analytical Chemistry, Department of Chemistry, University of Natural Resources and Life Sciences (BOKU) Vienna, Muthgasse 18, 1190, Vienna, Austria.

Analytical and Bioanalytical Chemistry
|October 13, 2021
PubMed
Summary
This summary is machine-generated.

We developed a novel yeast-derived reference material for metabolomics research. This biological matrix enables accurate metabolite quantification, enhancing research reliability and reproducibility.

Keywords:
Absolute quantificationHarmonizationMetabolomicsPichia pastorisReference materialTargeted analysis

More Related Videos

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.2K
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
08:56

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: March 8, 2020

7.5K

Related Experiment Videos

Last Updated: Oct 17, 2025

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
07:25

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: January 5, 2021

4.6K
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.2K
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
08:56

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: March 8, 2020

7.5K

Area of Science:

  • Biotechnology
  • Metabolomics
  • Analytical Chemistry

Background:

  • Metabolomics research requires reliable reference materials for accurate quantification.
  • Existing biological reference materials often lack comprehensive characterization or on-demand production capabilities.

Purpose of the Study:

  • To develop and validate a yeast-derived biological matrix reference material for metabolomics.
  • To establish a standardized method for producing and quantifying metabolites in the reference material.
  • To assess the homogeneity, stability, and repeatability of the developed reference material.

Main Methods:

  • Yeast (Pichia pastoris) cultivation using controlled fed-batch fermentation.
  • Standardized extraction protocols (cold methanol quenching, boiling ethanol extraction).
  • Absolute quantification of >70 primary metabolites using isotope dilution and mass spectrometry (HILIC-HRMS, GC-MS/MS).

Main Results:

  • Demonstrated feasibility of producing a defined yeast biomass reference material.
  • Achieved excellent homogeneity and stability over 6 months, with high repeatability over 2 years.
  • Quantified a panel of conserved primary metabolites using 13C-yeast internal standards.
  • Validation across two independent laboratories using complementary MS platforms.

Conclusions:

  • The developed yeast-derived reference material is suitable for metabolomics research.
  • The material demonstrates high quality, comparable or superior to existing standards.
  • This approach enables on-demand production of reliable biological matrix reference materials.