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Versatile Integration of Liquid-Phase Microextraction and Fluorescent Aptamer Beacons: A Synergistic Effect for
Bin Lin1, Huajing Liu1, Chuixiu Huang2
1Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection and State Key Laboratory of Environmental Health (Incubation), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road #13, Wuhan 430030, China.
A novel biosensor integrates liquid-phase microextraction with fluorescent aptamer beacons (FABs) for sensitive mercury detection in biological samples. This combined approach enhances sample purification and preconcentration, improving accuracy in real-world analyses.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Environmental Science
Background:
- Fluorescent aptamer beacons (FABs) are vital biosensors for environmental analysis.
- Common FABs exhibit low compatibility with biological samples, limiting their application.
- Current research focuses on complex FAB structures for biological sample adaptation.
Purpose of the Study:
- To develop an integrated biosensor system for analyzing biological samples.
- To overcome the limitations of traditional FABs in complex biological matrices.
- To enhance the sensitivity and accuracy of mercury ion (Hg2+) detection in urine.
Main Methods:
- Integration of flat membrane-based liquid-phase microextraction (FM-LPME) with FABs.
- Utilizing FM-LPME for sample cleanup and preconcentration of Hg2+.
- Employing FABs for sensitive recognition and detection of Hg2+.
Main Results:
- The integrated LPME-FAB system demonstrated synergistic enhancement in target analysis.
- LPME effectively purified and enriched Hg2+ for FAB recognition.
- FAB recognition improved the extraction efficiency of LPME.
- Achieved a detection limit of 27 nM for Hg2+ in urine samples with 98-113% accuracy.
Conclusions:
- The developed LPME-FAB system offers a novel and effective approach for Hg2+ analysis in biological samples.
- This study highlights the importance of integrating sample preparation with detection probes for advanced bioanalysis.
- The findings provide new insights for the next generation of biosensors.
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