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Updated: Oct 16, 2025

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

Scalable Nucleic Acid Storage and Retrieval Using Barcoded Microcapsules.

James L Banal¹, Mark Bathe^1,2

¹Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 United States.

ACS Applied Materials & Interfaces

|October 15, 2021

Summary

This summary is machine-generated.

A new method, Preservation and Access of Nucleic aciDs using barcOded micRocApsules (PANDORA), enables room-temperature storage of DNA and RNA. This approach allows for scalable, low-cost, and efficient retrieval of nucleic acid samples.

Keywords:

Genetics assays barcodes biopolymers silica sorting

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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Area of Science:

Biotechnology
Genomics
Molecular Biology

Background:

Advances in nucleic acid technologies necessitate efficient storage solutions.
Current low-temperature storage methods are costly and require robotics for access, limiting scalability.

Purpose of the Study:

To develop a scalable, low-cost, room-temperature method for nucleic acid storage and retrieval.
To present Preservation and Access of Nucleic aciDs using barcOded micRocApsules (PANDORA) as an alternative to conventional storage.

Main Methods:

Encapsulating nucleic acids (DNA/RNA) from various sources into silica microcapsules.
Utilizing molecular barcodes on microcapsules for sample identification and pooling.
Employing fluorescence-activated sorting for targeted nucleic acid retrieval.

Main Results:

Demonstrated quantitative storage and retrieval of nucleic acids from kilobases to gigabases.
Successfully recovered 100,000-200,000 samples with Boolean logic selection.
Validated sample integrity and sorting accuracy using quantitative PCR and sequencing.

Conclusions:

PANDORA offers a scalable, long-term, room-temperature storage solution for nucleic acids.
The method ensures high sample fidelity and efficient retrieval.
This technology addresses the limitations of current low-temperature storage systems.