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Conditional and tissue-specific approaches to dissect essential mechanisms in plant development
Marie L Pfeiffer1, Joanna Winkler1, Daniël Van Damme1
1Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent, Belgium; VIB Center for Plant Systems Biology, 9052, Ghent, Belgium.
Current Opinion in Plant Biology
|October 15, 2021
Summary
CRISPR technology enables precise gene editing in plants, overcoming lethal mutations. This review covers new DNA, RNA, and protein manipulation methods for conditional gene function studies.
Area of Science:
- Plant molecular biology
- Genetics and genomics
Background:
- Reverse genetics is key for gene function studies.
- Lethal mutations limit traditional methods.
- Spatial and temporal control is needed for gene function analysis.
Purpose of the Study:
- To review advancements in plant gene manipulation.
- Focus on tissue-specific and conditional approaches.
- Covering DNA, RNA, and protein level modifications.
Main Methods:
- CRISPR-Cas9 technology for targeted genome editing.
- CRISPR-based strategies for RNA manipulation.
- Novel protein-level manipulation techniques in plants.
Main Results:
- CRISPR has revolutionized plant genome editing.
- Enabled inducible and tissue-specific gene function studies.
- New protein-targeting methods expand functional analysis.
Conclusions:
- CRISPR technologies offer powerful tools for plant research.
- Conditional and targeted approaches overcome limitations of lethal mutations.
- Advances in DNA, RNA, and protein manipulation enhance gene function discovery.
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