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Discovering Myeloid Cell Heterogeneity in Mandibular Bone - Cell by Cell Analysis
Kyu Hwan Kwack1, Natalie A Lamb2, Jonathan E Bard2,3
1Department of Oral Biology, University at Buffalo, The State University of New York, Buffalo, NY, United States.
Frontiers in Physiology
|October 18, 2021
Summary
Mandibular bone marrow has fewer myeloid cells but increased immunosuppressive activity compared to long bone. These site-specific differences in myeloid progenitor populations and gene expression highlight bone marrow heterogeneity.
Area of Science:
- Immunology
- Cell Biology
- Genomics
Background:
- Myeloid-derived bone marrow progenitor populations exhibit varying osteoclastogenesis potential based on anatomical location.
- Myeloid progenitors from long bones (femur, tibia) show greater osteoclast differentiation potential than those from alveolar bone (mandible).
Purpose of the Study:
- To investigate the distinct myeloid lineage progenitor cell populations in mandibular bone versus long bone.
- To provide a comprehensive transcriptional landscape of these cell populations using advanced techniques.
Main Methods:
- Flow cytometry was employed to analyze cell populations.
- High-throughput single-cell RNA sequencing (scRNA-seq) was utilized for transcriptional profiling.
- Analysis focused on myeloid-derived suppressor cell (MDSC)-like populations, macrophages, hematopoietic stem cells, and progenitor populations.
Main Results:
- Mandibular bone marrow showed deficits in myeloid differentiation, with fewer MDSC-like cells and macrophages, despite increased MDSC immunosuppressive activity.
- No defects were observed in hematopoietic stem cell populations (LSK, LK) in mandibular bone.
- Granulocyte-monocyte and monocyte progenitors were decreased in mandibular bone marrow, while regulatory T lymphocytes and B lymphocytes were increased.
Conclusions:
- Mandibular bone marrow exhibits unique myeloid progenitor cellular composition and distinct transcriptional programs compared to long bone.
- These findings reveal site-specific heterogeneity in myeloid cell populations, impacting our understanding of bone marrow biology.
