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Updated: Oct 16, 2025

Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

Published on: June 19, 2021

Studying the Tumor Microenvironment in Zebrafish.

Caterina Sturtzel¹, Jennifer Hocking², Stefanie Kirchberger¹

¹St. Anna Children's Cancer Research Institute, Innovative Cancer Models, Vienna, Austria.

Advances in Experimental Medicine and Biology

|October 19, 2021

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Zebrafish intravital imaging reveals tumor microenvironment cell roles in cancer initiation and metastasis. This model aids understanding of immune cells, adipocytes, fibroblasts, and endothelial cells for new cancer therapies.

Area of Science:

Oncology
Developmental Biology
Immunology

Background:

The tumor microenvironment (TME) is crucial for cancer progression, including initiation, angiogenesis, and metastasis.
Understanding TME cellular players is key to developing novel cancer therapeutics.
Intravital imaging offers powerful insights into tumor cell interactions.

Purpose of the Study:

To summarize the utility of zebrafish as a model organism for studying the TME.
To highlight how zebrafish facilitates in vivo characterization of TME cells.
To review research on various TME components using zebrafish.

Main Methods:

Utilizing zebrafish for intravital imaging studies of the TME.
Employing both genetically engineered and xenograft cancer models in zebrafish.

Keywords:

Adipocytes Cancer Compound testing Extracellular vesicles Fibroblasts In vivo Innate immune cells Live imaging Macrophages Metastasis Microglia Neo-angiogenesis Neutrophils Tumor microenvironment Tumor proliferation Zebrafish

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Related Experiment Videos

Last Updated: Oct 16, 2025

Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

Published on: June 19, 2021

Zebrafish Model of Neuroblastoma Metastasis

Zebrafish Model of Neuroblastoma Metastasis

Published on: March 14, 2021

Author Spotlight: Advancing Cancer Therapeutics Using Tumor Xenotransplantation in Zebrafish

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Published on: July 12, 2024

Analyzing the roles of innate immune cells, adipocytes, fibroblasts, and endothelial cells.

Main Results:

Zebrafish models have been instrumental in dissecting the roles of innate immune cells in tumor initiation and metastasis.
Studies in zebrafish have also elucidated the functions of adipocytes, fibroblasts, and endothelial cells within the TME.
The zebrafish model allows for dynamic, in vivo observation of cellular interactions within the TME.

Conclusions:

Zebrafish is a versatile and powerful model for in vivo TME research.
Intravital imaging in zebrafish provides critical insights into TME biology.
This research supports the development of targeted cancer therapies by clarifying TME cell functions.