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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Oct 16, 2025

NanoDrop Microvolume Quantitation of Nucleic Acids

NanoDrop Microvolume Quantitation of Nucleic Acids

Published on: November 22, 2010

Cq Values Do Not Reflect Nucleic Acid Quantity in Biological Samples

J M Ruijter¹, A Ruiz-Villalba², M J B van den Hoff¹

¹Department of Medical Biology, Amsterdam University Medical Centres, Location AMC, Amsterdam, the Netherlands.

Clinical Chemistry

|October 22, 2021

Summary

No abstract available in PubMed .

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