Acorn Ants May Create and Use Two Entrances to the Nest Cavity

Many ant species construct large nests that are inhabited by numerous workers, but other species dwell in ready-for-use cavities and live in small colonies. Ants of the genus Temnothorax inhabit small cavities, e.g., in acorns, twigs, and under rocks. Although a preference for nest sites with a narrower entrance is known, recent studies have shown that they also use cavities with wider entrances and may modify the size of such entrances. As good cavities for nest sites are a limited resource, the possibility to modify a potential nest site, including a reduction in the size of the hole, should be a favorable matter for the ants. Through field and laboratory experiments, I studied the acorn ant Temnothorax crassispinus. Observations showed that they readily inhabited imperfect cavities and, if necessary, modified the holes to such cavities. If they had to repair a nest site, they sometimes created a second entrance; there was no difference in the sizes of the entrances. In the field, for entrance modification or blocking an unnecessary hole, the acorn ants used soil, grains of sand, and parts of plants. In the laboratory, the ant colonies showed no preference for nest sites with one entrance vs. a nest cavity with two entrances. The results of this study showed that even such small ant colonies could use nest sites with multiple entrances; however, the effect of the presence of more than one entrance on the behavior of the ants is unknown.