Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Viruses with RNA Genomes01:29

Viruses with RNA Genomes

229
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
229
Replication in Prokaryotes02:35

Replication in Prokaryotes

91.2K
Overview
91.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intersegmental transfers drive target search in an RNA-targeting CRISPR system.

bioRxiv : the preprint server for biology·2026
Same author

Spatio-temporal modelling of in vitro influenza A virus infection: The impact of defective interfering particles on the type I interferon response.

PLoS computational biology·2026
Same author

Polymerase trapping as the mechanism of H5 highly pathogenic avian influenza virus genesis.

Science (New York, N.Y.)·2026
Same author

Febrile temperature activates the innate immune response by promoting aberrant influenza A virus RNA synthesis.

Science advances·2026
Same author

Spatio-temporal modelling of <i>in vitro</i> influenza A virus infection: the impact of defective interfering particles on type I interferon response.

bioRxiv : the preprint server for biology·2025
Same author

Amplification-free detection of zoonotic viruses using Cas13 and multiple CRISPR RNAs.

The Journal of general virology·2025

Related Experiment Video

Updated: Oct 15, 2025

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
15:57

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

22.7K

Understanding viral replication and transcription using single-molecule techniques.

Emmanuelle Pitre1, Aartjan J W Te Velthuis2

  • 1Division of Virology, Department of Pathology, University of Cambridge, Cambridge, United Kingdom.

The Enzymes
|October 26, 2021
PubMed
Summary

Single-molecule techniques reveal crucial details about viral enzymes, aiding antiviral drug development. These methods illuminate enzyme function, interactions, and mechanisms, advancing our understanding of virus replication.

Keywords:
DNAHelicaseRNARNA polymeraseSingle-strand binding protein

More Related Videos

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

18.9K
Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization
07:37

Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization

Published on: September 27, 2024

1.9K

Related Experiment Videos

Last Updated: Oct 15, 2025

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
15:57

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

22.7K
Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

18.9K
Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization
07:37

Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization

Published on: September 27, 2024

1.9K

Area of Science:

  • Virology
  • Biochemistry
  • Molecular Biology

Background:

  • Viral enzymes like polymerases are essential for replication and key targets for antiviral therapies.
  • Understanding these enzymes' functions and interactions is critical for developing effective treatments.

Purpose of the Study:

  • To explore how various single-molecule approaches can elucidate viral enzyme mechanisms.
  • To highlight the insights gained into viral replication complexes and antiviral drug actions.

Main Methods:

  • Single-molecule Förster Resonance Energy Transfer (smFRET)
  • Magnetic tweezers
  • Optical tweezers
  • Atomic force microscopy
  • Flow stretching

Main Results:

  • Single-molecule methods provide insights into enzyme step-sizes, activity heterogeneity, and conformational dynamics.
  • These techniques reveal details about polymerase fidelity and the mechanics of antiviral drug action.
  • Novel understanding of protein choreography within viral replication complexes has been achieved.

Conclusions:

  • Single-molecule biophysics offers powerful tools for dissecting complex viral enzyme mechanisms.
  • These advanced techniques are instrumental in understanding viral replication and guiding antiviral drug discovery.