Impact of Seminal Plasma Composition on Sperm Freezability in Wild Mammals: A Review
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Updated: Oct 14, 2025

Sperm Collection of Differential Quality Using Density Gradient Centrifugation
Published on: November 29, 2018
Samara Sandy Jerônimo Moreira1, Arthur Emannuel de Araújo Lago1, Arlindo Alencar Araripe Moura2
1Laboratory for Animal Germplasm Conservation, Department of Animal Sciences, Federal University of the Semiarid Region, Mossoró, Brazil.
Abstract:
This review was designed to summarize the most important information around seminal plasma composition and discuss its impact on the freezability of wild mammal semen samples. Seminal plasma is made up of various biochemical constituents, including ions, lipids, proteins, enzymes, and sugars, which vary between species in response to the presence and size of any relevant accessory glands. The biochemical constituents of seminal plasma may change as a result of age, individual variability, and seasonality. These constituents are responsible for supporting different functions in sperm cells, contributing to motility, acrosomal reaction, and fertilization events. A detailed understanding of seminal plasma biochemistry may help to optimize semen freezing protocols, enabling the dynamic alteration in diluents to allow for increased sperm viability rates after thawing.
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