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Effect of Radiotherapy and Low-Level Laser Therapy on Circulating Blood Cells of Rats.
Milene Castilhos de Oliveira1, Gabriel Francisco Krueger1, Juliana Tomaz Sganzerla1
1Department of Oral Medicine, Graduate Program in Dentistry, Universidade Luterana do Brasil, Canoas, RS, Brazil.
Journal of Lasers in Medical Sciences
|November 4, 2021
Summary
Low-level laser therapy (LLLT) may positively impact red blood cell counts following VMAT radiotherapy in rats. This therapy shows potential systemic effects on blood cell counts, irrespective of timing relative to radiation exposure.
Area of Science:
- Biomedical Engineering
- Radiation Oncology
- Laser Therapy
Background:
- Volumetric modular arc therapy (VMAT) is a radiotherapy technique that can cause side effects.
- Blood cell count alterations are a known consequence of radiotherapy.
- Low-level laser therapy (LLLT) is being explored for its potential therapeutic effects.
Purpose of the Study:
- To investigate the impact of LLLT on blood cell counts in rats subjected to VMAT.
- To determine if the timing of LLLT application (immediate vs. delayed) influences its effect on blood cell parameters.
Main Methods:
- Wistar rats were divided into control, immediate LLLT (24h post-VMAT), and delayed LLLT (120h post-VMAT) groups.
- VMAT was administered to laser groups at a single dose of 12 Gy.
- LLLT (660 nm, 100 mW) was applied to parotid glands for 10 days.
- Blood samples were analyzed for erythrogram, leukogram, and platelet counts.
Main Results:
- LLLT groups exhibited higher red blood cell counts compared to controls, particularly the delayed group.
- Hematimetric indices (MCV, MCH) were lower in LLLT groups, especially the delayed group.
- White blood cell counts decreased in the immediate LLLT group, primarily due to reduced lymphocytes.
Conclusions:
- LLLT demonstrates a potential systemic effect on circulating red blood cell counts.
- The timing of LLLT application (immediate or delayed) does not negate its positive influence on red blood cells post-radiotherapy.
