Related Experiment Video
Updated: Oct 14, 2025

13:02
Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography TLC Coupled with Gas-Liquid Chromatography GLC
Published on: March 18, 2011
37.3K
[STUDY OF LIPIDS AND ACCOMPANYING BIOLOGICALLY ACTIVE COMPOUNDS OF SOME PLANTS GROWING GEORGIA]
B Kikalishvili1, Ts Sulakvelidze1, M Malania1
1Tbilisi State Medicae University, I. Kutateladze Institude of Pbarmacicochemistry, Georgia.
Georgian Medical News
|November 8, 2021
Summary
This study analyzed lipids and bioactive compounds in St. John's Wort, corn, and horsetail. Key findings include identifying fatty acids, phospholipids, carotenoids, and amino acids in these plant materials.
Area of Science:
- Phytochemistry
- Analytical Chemistry
- Plant Science
Background:
- Plant-derived compounds are crucial in traditional medicine and modern pharmacology.
- Understanding the biochemical composition of medicinal plants like Hypericum perforatum, Zea mays, and Equisetum arvense is essential for their effective utilization.
- Lipids and bioactive substances contribute significantly to the therapeutic properties of plants.
Purpose of the Study:
- To quantify the lipid content and identify bioactive compounds in seeds of Hypericum perforatum L. and Zea mays L., and in the above-ground parts of Equisetum arvense L.
- To characterize the physical-chemical properties of extracted lipid fractions.
- To explore the presence of specific classes of lipids and other bioactive molecules.
Main Methods:
- Extraction of neutral and polar lipid sums from plant materials.
- Analysis of physical-chemical constants.
- Gas Chromatography-Mass Spectrometry (GC-MS) for qualitative and quantitative analysis.
- Identification of fatty acids, phospholipids, carotenoids, and amino acids.
Main Results:
- Lipid sums (neutral and polar) were successfully obtained with significant yields.
- Neutral lipid fractions were rich in saturated, unsaturated, and polyunsaturated fatty acids.
- Polar lipid fractions confirmed the presence of phospholipids.
- Carotenoids and amino acids were identified as significant bioactive compounds.
Conclusions:
- The investigated plant materials (Hypericum perforatum, Zea mays, Equisetum arvense) possess valuable lipid profiles, including diverse fatty acids and phospholipids.
- These plants are sources of important bioactive compounds such as carotenoids and amino acids.
- The findings support the potential use of these plants in pharmaceutical and nutraceutical applications.
Related Concept Videos
What are Lipids?
212.1K
Overview
212.1K
Biosynthesis of Lipids
165
Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis...
165
Lipid Catabolism
281
Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
281
Lipid-derived Compounds in the Human Body
5.5K
Fats and lipids are crucial components in the human body. Some lipid-derived compounds, such as fat-soluble vitamins, eicosanoids, lipoproteins, and glycolipids, also play unique roles to support various biological processes .
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin,...
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin,...
5.5K
Lipid Digestion
95.2K
Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific steps the body must take to break down lipids and make them available for use.
95.2K
Structure of Lipids
12.6K
12.6K

