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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
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Related Experiment Video

Updated: Oct 14, 2025

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
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Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

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Biotinylation-based proximity labelling proteomics: basics, applications and technical considerations.

Tomoya Niinae, Yasushi Ishihama, Koshi Imami

    Journal of Biochemistry
    |November 9, 2021
    PubMed
    Summary
    This summary is machine-generated.

    Proximity labeling (PL) with biotinylation maps cellular proteins and interactions in living systems. This review covers PL proteomics basics, applications, and advanced enrichment techniques for proteins and peptides.

    Keywords:
    affinity purificationbiotinylation proteininteractionproteomicsproximity labelling

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    Area of Science:

    • Cellular and Molecular Biology
    • Proteomics
    • Biochemistry

    Background:

    • Biotinylation-based proximity labeling (PL) enables high-resolution mapping of cellular components.
    • PL combined with mass spectrometry is a powerful tool for studying molecular interactions and proteomes.

    Purpose of the Study:

    • To review the fundamentals and biological applications of proximity labeling proteomics.
    • To highlight recent advancements in enrichment techniques for biotinylated proteins and peptides.

    Main Methods:

    • Proximity labeling coupled with mass spectrometry-based proteomics.
    • Review of existing literature on PL techniques and applications.

    Main Results:

    • PL has been successfully applied to identify protein-protein interactions, protein-nucleic acid interactions, organelle proteomes, and secretomes.
    • Recent developments focus on improved enrichment strategies for biotinylated molecules.

    Conclusions:

    • Proximity labeling offers significant advantages for studying cellular organization and molecular interactions in vivo.
    • Continued development of enrichment techniques will further enhance the utility of PL proteomics.