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Updated: Oct 13, 2025
Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
Published on: June 23, 2016
Dual-wavelength responsive photoelectrochemical aptasensor based on ionic liquid functionalized Zn-MOFs and noble
Yingying Zhong1, Xian Wang1, Ruyan Zha1
1Key Laboratory of Catalysis and Energy Materials Chemistry of Ministry of Education & Hubei Key Laboratory of Catalysis and Materials Science & Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, South-Central University for Nationalities, Wuhan 430074, China. wangyychem@mail.scuec.edu.cn.
Abstract:
A single tumor marker may correspond to a variety of diseases, and a specific disease requires the joint detection of multiple tumor markers for improving the accuracy of diagnoses. An ionic liquid-functionalized metal-organic framework (Zn-MOF microspheres) was used as the substrate to capture the aptamer (Ab1), and noble metal nanoparticles were used to label a signal aptamer (Ab2) to construct a dual-wavelength responsive sandwich-type photoelectrochemical (PEC) aptasensor. Due to the size effect, plasma resonance and the response of the noble metal nanoparticle enhancement system to different excitation wavelengths, the simultaneous detection of CEA and CA153 tumor markers was realized. Under the optimized conditions, CA153 and CEA at concentrations of 0.05-100 U mL-1 and 0.005-10 ng mL-1 were detected by the PEC aptasensor. Detection limits calculated for CA153 and CEA determinations were 0.0275 U mL-1 and 2.85 pg mL-1 (S/N = 3), respectively. CA153 and CEA in serum samples were detected by the PEC aptasensor, and their concentrations were well consistent with that obtained from the ELISA. In addition, the PEC aptasensor exhibited a recovery rate of 96.98%-103.4%, and a relative standard deviation of 1.1%-3.6%, indicating good practical value and accuracy, further confirming its potential for clinical diagnosis.
