Dense Packed Drivable Optrode Array for Precise Optical Stimulation and Neural Recording in Multiple-Brain Regions.

Area of Science:

• Neuroscience
• Biomedical Engineering
• Electrophysiology

Background:

• Neural network complexity hinders complete understanding.
• Existing planar probes have limitations in recording scope and simultaneous multi-region monitoring.
• Advanced tools are needed for comprehensive neural circuit studies.

Purpose of the Study:

• To develop a 3D high-density drivable optrode array for simultaneous, large-scale neural recording and precise optical stimulation.
• To overcome the limitations of traditional planar probes for neural circuit research.

Main Methods:

• Designed a 3D optrode array with four-layer probes, 1024 microelectrodes, and two optical fibers in a drivable module.
• Optimized microelectrode recording performance via electrochemical modification.
• Verified precise implantation depth control and conducted in vivo experiments.

Main Results:

• Successfully monitored neural activities in CA1 and dentate gyrus regions.
• Achieved neuron firing tracking for two weeks.
• Demonstrated optogenetic control by suppressing neuron firing using blue light via high-density optrodes.

Conclusions:

• The 3D high-density drivable optrode array offers large-scale recording and optoelectronic integration capabilities.
• This technology is valuable for advancing research in neural networks and brain diseases.
• Enables simultaneous multi-brain region recording and precise optical stimulation.