Related Experiment Video
Updated: Oct 13, 2025
12:20
Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
Published on: August 6, 2014
11.9K
Visible light-activatable Q-dye molecular beacons for long-term mRNA monitoring in neurons
Robin Klimek1, Paul G Donlin-Asp2, Claudio Polisseni2
1Institute of Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, Max-von-Laue-Straße 9, Frankfurt am Main 60438, Germany. heckel@uni-frankfurt.de.
Chemical Communications (Cambridge, England)
|November 15, 2021
Abstract:
Herein, we present a new class of Q-dye molecular beacons (MBs) that can be locally activated with visible light in hippocampal neurons. Our novel architecture increases the available monitoring time for neuronal mRNA from several minutes to 14 hours, since a lower light-sampling rate is required for tracking.
Related Concept Videos
03:31Labeling DNA Probes
8.6K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.6K
02:11Reporter Genes
12.1K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
12.1K