Multichannel dual protein sensing using amphiphilic supramolecular assemblies
Jingjing Gao1, Theeraphop Prachyathipsakul1, S Thayumanavan1
1Department of Chemistry, University of Massachusetts Amherst, MA, 01002, USA. thai@umass.edu.
Abstract:
Protein sensing strategies have implications in detection of many human pathologies. Here, a supramolecular strategy for sensing two different proteins using a multichannel readout approach is outlined. Protein-ligand binding or enzymatic cleavage can both be programmed to induce supramolecular disassembly, which leads to fluorescence enhancement via aggregation-induced emission (AIE), protein-induced fluorescence enhancement (PIFE), or disassembly-induced fluorescence enhancement (DIFE). The accompanying signal change from two different fluorophores and their patterns are then used for specific protein sensing.
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