Related Experiment Video
Updated: Oct 13, 2025

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
Published on: August 23, 2024
Electrotransformation of thermophilic bacterium Caldimonas manganoxidans
Takamitsu Arai1, Shimpei Aikawa1, Kumar Sudesh2
1Japan International Research Center for Agricultural Sciences, 1-1 Ohwashi Tsukuba, Ibaraki 305-8686, Japan.
Abstract:
Caldimonas manganoxidans is a Gram-negative, thermophilic, bioplastic-producing bacterium that is a promising strain to overcome the drawbacks of existing bioplastic manufacturing methods. However, genetic manipulation of this species has not previously been studied. Here, we developed an optimized electrotransformation protocol for C. manganoxidans by screening conditions, including the bacterial growth phase, electroporation buffer, pulse strength, and recovery time. The optimized transformation protocol obtained (3.1 ± 0.78) × 108 colony-forming units/μg DNA of plasmid pBBR1MCS-2. High transformation efficiency was observed when using plasmid DNA isolated from C. manganoxidans. The DNA methylases of Escherichia coli did not affect the transformation efficiency of C. manganoxidans. The electrotransformation technique proposed here will be beneficial for the genetic manipulation of thermophilic Caldimonas species.
Related Concept Videos
Hyperthermophilic Bacteria
Metabolism of Chemolithotrophs
Anoxygenic Phototrophic Bacteria
Microbial Nutrition
Transformation
Diversity of Archaea IV

