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Updated: Oct 12, 2025

Small-Scale Extraction of Caenorhabditis elegans Genomic DNA
Published on: June 7, 2022
The genome sequence of the Australian filarial nematode, Cercopithifilaria johnstoni
Kirsty McCann1, Warwick Grant1, Stephen R Doyle2
1Department of Physiology, Anatomy & Microbiology, La Trobe University, Bundoora, Australia.
Abstract:
We present a genome assembly and annotation of an individual female Cercopithifilaria johnstoni, a parasitic filarial nematode that is transmitted by hard ticks (Ixodidae) to infect a broad range of native Australian murid and marsupial hosts. The genome sequence is 76.9 Mbp in length, and although in draft form (N50 = 99 kbp, N50[n] = 232), is largely complete based on universally conserved orthologs (BUSCOs; genome = 94.9%, protein = 96.5%) and relative to other related filarial species. These data represent the first genomic resources for the genus Cercopithifilaria, a group of parasites with a broad host range, and form the basis for comparative analysis with the human-infective parasite, Onchocerca volvulus, both of which are responsible for similar eye and skin pathologies in their respective hosts.
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