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Modern phosphotriester synthesis of long oligonucleotides and their use in genetic engineering
O G Chakhmakhcheva1, A A Buryakova, V A Efimov
1Shemyakin Institute of Bio-organic Chemistry, USSR Academy of Sciences, Moscow.
Nucleic Acids Symposium Series
|January 1, 1987
Abstract:
Long oligodeoxyribonucleotides synthesized by the modern phosphotriester method, which is based on the use of O-nucleophilic intramolecular catalysis, were applied for the construction of artificial DNAs and directed mutagenesis of some genes.