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Related Experiment Videos

Round window membrane permeability. An in vitro model.

L Lundman1, D Bagger-Sjöbäck, L Holmquist

  • 1Department of Otorhinolaryngology, Karolinska Hospital, Stockholm, Sweden.

Acta Oto-Laryngologica. Supplementum
|January 1, 1987
PubMed
Summary
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This study validates an in vitro round window membrane model for assessing ototoxic substance passage. The model accurately measures permeability, crucial for understanding hearing loss in middle ear infections.

Area of Science:

  • Otic physiology
  • Membrane transport
  • Auditory research

Background:

  • The round window membrane (RWM) is a key pathway for ototoxic substances entering the inner ear.
  • Sensorineural hearing impairment is a clinical concern in otitis media.
  • Understanding RWM permeability is vital for ototoxicity research.

Purpose of the Study:

  • To evaluate the accuracy and reliability of an in vitro model for RWM permeability.
  • To quantify the passage of different molecular weight substances across the RWM.
  • To establish a controlled method for studying RWM transport.

Main Methods:

  • Resection of the mongolian gerbil RWM with its bone niche.
  • Mounting the RWM preparation between two chambers simulating middle ear and perilymphatic spaces.

Related Experiment Videos

  • Measuring the passage of low-density lipoprotein, high-density lipoprotein, and horseradish peroxidase.

    • Main Results:

    • No passage of low-density lipoprotein (2,300 kD) or high-density lipoprotein (115-350 kD) through the RWM within 3 hours.
    • Horseradish peroxidase (40 kD) passage rate of 6 µg/h at a concentration of 10 g/l.
    • The in vitro model demonstrated no leakage artifacts between chambers.

    Conclusions:

    • The developed in vitro model is accurate and reliable for studying RWM permeability.
    • This model allows for controlled measurement of substance passage across the RWM.
    • Findings are significant for understanding ototoxicity and developing protective strategies.