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In Silico Prediction Methods for Site-Saturation Mutagenesis.

Ge Qu1,2, Zhoutong Sun3,4

  • 1Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.

Methods in Molecular Biology (Clifton, N.J.)
|November 23, 2021
PubMed
Summary

Computational methods guide protein engineering by predicting optimal amino acid changes for biocatalysts. This approach enhances enzyme function and specificity, reducing extensive screening in directed evolution for industrial applications.

Keywords:
Alcohol dehydrogenaseCarboxylic acid reductaseConformational dynamicsEnzyme engineeringIn silicoProtein–ligand interactionRational designSite-specific saturation mutagenesis

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