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Updated: Oct 12, 2025

Reproductive Techniques for Ovarian Monitoring and Control in Amphibians
Published on: May 12, 2019
Sedation and Anesthesia of Amphibians
Peter M DiGeronimo1, Julie A Balko2
1Adventure Aquarium, 1 Riverside Drive, Camden, NJ 08103, USA; Animal & Bird Health Care Center, 1785 Springdale Road, Cherry Hill, NJ 08003, USA.
Abstract:
Amphibians commonly are managed under human care for research, education, conservation, and companionship and frequently are in need of sedation, anesthesia, or end-of-life care involving euthanasia. Objective investigation of sedative and anesthetic protocols in these taxa still is in its infancy, but knowledge of current best practices is paramount to appropriate care. Tricaine methanesulfonate delivered via immersion (bath) is the most common anesthetic agent in amphibians, but several other effective techniques have been identified. This summary provides a comprehensive review of the current evidence-based literature regarding amphibian sedative, anesthetic, and euthanasia techniques.
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