Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Characterization and modulation of human insulin degrading enzyme conformational dynamics to control enzyme activity.

eLife·2026

Same author

Characterization Standard for <i>In-situ</i> Cryo-electron Tomography.

bioRxiv : the preprint server for biology·2026

Same author

AreTomoLive: automated reconstruction of comprehensively corrected and denoised cryo-electron tomograms in real time and at high throughput.

Nature methods·2026

Same author

copick: An open dataset interface and toolkit for collaborative annotation and analysis of cryo-electron tomography data.

Protein science : a publication of the Protein Society·2026

Same author

Evaluating the Volta phase plate for improved tomogram alignment in cryo-electron tomography.

IUCrJ·2026

Same author

Editorial: Breakthroughs in Cryo-EM with machine learning and artificial intelligence.

Frontiers in molecular biosciences·2025

Search research articles

Related Experiment Video

Updated: Oct 12, 2025

Author Spotlight: Enhancing Cryo-Electron Microscopy by Automated Data Collection and Analysis Techniques

Author Spotlight: Enhancing Cryo-Electron Microscopy by Automated Data Collection and Analysis Techniques

Published on: December 1, 2023

Broadening access to cryoEM through centralized facilities.

Christina M Zimanyi¹, Mykhailo Kopylov¹, Clinton S Potter²

¹Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.

Trends in Biochemical Sciences

|November 26, 2021

Summary

This summary is machine-generated.

Cryogenic electron microscopy (cryoEM) provides high-resolution macromolecular structures from frozen samples. This technology is rapidly advancing biomedical research, drug discovery, and vaccine development through accessible national centers.

Keywords:

automation biophysics education cryogenic electron microscopy (cryoEM)structural biology training

More Related Videos

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

Related Experiment Videos

Last Updated: Oct 12, 2025

Author Spotlight: Enhancing Cryo-Electron Microscopy by Automated Data Collection and Analysis Techniques

Author Spotlight: Enhancing Cryo-Electron Microscopy by Automated Data Collection and Analysis Techniques

Published on: December 1, 2023

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

Area of Science:

Structural biology
Biomedical research
Drug discovery

Background:

Cryogenic electron microscopy (cryoEM) enables high-resolution structural analysis of biological specimens.
It offers insights into previously inaccessible levels of biological organization.
Recent technological advances have accelerated molecular and cellular structure determination.

Purpose of the Study:

To highlight the broad impact of cryoEM in biomedical research.
To discuss the role of national and regional centers in supporting cryoEM growth.
To emphasize the establishment of sustainable workflows and research capacity.

Main Methods:

Utilizes images of frozen hydrated biological specimens.
Leverages advanced imaging and reconstruction techniques.
Involves the establishment of national and regional cryoEM centers.

Main Results:

CryoEM is opening up new levels of biological organization to high-resolution analysis.
Significant expansion in molecular and cellular structure determination is occurring.
National and regional centers are increasing accessibility and promoting best practices.

Conclusions:

CryoEM has transformative potential across basic biology, drug discovery, and vaccine development.
A network of centers is crucial for accelerating adoption and building research capacity.
Synergy among centers ensures sustainable workflows and future advancements.