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Updated: Oct 11, 2025

A Polyaniline-based Sensor of Nucleic Acids
Published on: November 1, 2016
Pyridinium-conjugated polynorbornenes for nanomolar ATP sensing using an indicator displacement assay and a PET
Ling-Xi Huang1, Yue-Bo He1, Jaewon Kim2
1Department of Chemistry, Nanchang University, Nanchang 330031, P. R. China. cqyong@ncu.edu.cn.
Abstract:
An indicator displacement assay, namely polymeric PNPY-n/UD consisting of a cationic polynorbornene backbone with pyridinium functional groups (PNPY-1,2,3) and an anionic uranine dye (UD) as an indicator, has been developed for highly sensitive "turn-on" fluorescence sensing of ATP. While PNPY-1/UD itself is non-emissive, a bright green fluorescence signal was observed in the presence of ATP [Ka = 2.17 × 105 M-1, LOD = 5.7 nM]. The potential of a highly photostable system PNPY-1/UD was also validated in detecting ATP levels in live-cell imaging applications.

