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Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Published on: January 3, 2015

Towards precise large genomic fragment deletion.

Xiangyang Li¹, Xingxu Huang²

¹Gene Editing Center, School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.

Trends in Genetics : TIG

|December 7, 2021

Summary

This summary is machine-generated.

Prime editing efficiently deletes large DNA fragments. This versatile gene editing tool offers precise large-scale genomic modifications beyond base substitutions.

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Area of Science:

Molecular Biology
Genetics
Gene Editing Technologies

Background:

Prime editing (PE) is a powerful gene editing system.
PE enables precise installation of small insertions, deletions, and base substitutions.

Purpose of the Study:

To investigate the capability of prime editing for large DNA fragment deletions.
To assess the efficiency and precision of PE in large-scale genomic modifications.

Main Methods:

Utilizing engineered prime editing systems.
Employing molecular assays to detect and quantify large DNA deletions.
Analyzing the precision of the deletion process.

Main Results:

Demonstrated that prime editing can be optimized for efficient deletion of large DNA fragments.
Showcased the precision of prime editing in executing these large-scale genomic alterations.
Confirmed the versatility of prime editing beyond small edits.

Conclusions:

Prime editing is a highly adaptable tool for precise large DNA fragment deletion.
This advancement expands the utility of prime editing for complex genomic engineering applications.
Further research can explore additional large-scale editing capabilities of prime editing.