Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

7.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
7.8K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.2K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.2K
Conserved Binding Sites01:49

Conserved Binding Sites

4.6K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Glycocalyx micro- and nanodomains in cell-cell and cell-matrix interactions revealed by enhanced click chemistry.

Nature communications·2026
Same author

Terminal Conjugation Enables Nanopore Sequencing of Peptides.

Journal of the American Chemical Society·2026
Same author

Temperature-dependent reaction rates of quinone-alkene cycloaddition reveal that only entropy determines the rate of SPOCQ reactions.

Chemical science·2025
Same author

Synthesis and biological evaluation of thiosemicarbazone-based antibody-drug conjugates.

RSC medicinal chemistry·2025
Same author

Resolving Sulfation Posttranslational Modifications on a Peptide Hormone using Nanopores.

ACS nano·2024
Same author

Modular Semisynthetic Approach to Generate T Cell-Dependent Bispecific Constructs from Recombinant IgG1 Antibodies.

Bioconjugate chemistry·2024
Same journal

Fabrication of an Antibacterial Alginate/Chitosan Hydrogel Dressing Loaded With CuO Nanoparticles for Wound Dressing Applications.

Biopolymers·2026
Same journal

Effect of Chitosan-Alginate Polyelectrolyte Complex Formation and Multilayer Polymer Configuration on the Characteristics of 3D-Printed Metronidazole-Loaded Periodontal Films.

Biopolymers·2026
Same journal

Phenolic Grafting of Oxidized Cellulose Nanofibers Using Ferulic Acid: Structural and Antioxidant Analysis Toward Bioactive Nanomaterials.

Biopolymers·2026
Same journal

Detection of a Target Nucleic Acid by Ligation-Assisted Fluorescence Enhancement of a Peptide Nucleic Acid (PNA) Twin Probe via Disulfide Binding.

Biopolymers·2026
Same journal

Influence of the Adsorption Time on the Growth of Bovine Serum Albumin-Chondroitin Sulfate Multilayer Films: A Surface Plasmon Resonance Spectroscopy Study.

Biopolymers·2026
Same journal

Influence of Boron Nitride Nanosheets on the Properties of Gelatin-Chitosan Bioinks for Extrusion-Based 3D Bioprinting.

Biopolymers·2026
See all related articles

Related Experiment Video

Updated: Oct 10, 2025

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications
10:12

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

3.1K

DNA-assisted site-selective protein modification.

Jordi F Keijzer1, Bauke Albada1

  • 1Laboratory of Organic Chemistry, Wageningen University and Research, Wageningen, The Netherlands.

Biopolymers
|December 8, 2021
PubMed
Summary
This summary is machine-generated.

DNA enhances protein modification for research and therapeutics. It acts as a template, guidance system, and catalyst, improving efficiency and site selectivity in biochemical reactions.

Keywords:
DNA chemistryDNAzymesaptamersbioconjugation chemistryprotein conjugates

More Related Videos

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation
09:12

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Published on: September 16, 2019

8.4K
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

9.6K

Related Experiment Videos

Last Updated: Oct 10, 2025

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications
10:12

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

3.1K
DNAzyme-dependent Analysis of rRNA 2’-O-Methylation
09:12

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Published on: September 16, 2019

8.4K
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

9.6K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Protein modification is crucial for proteomics and therapeutic development.
  • Existing protein modification methods often lack efficiency and site selectivity.
  • Novel strategies are needed to improve accuracy and yield in protein modification.

Purpose of the Study:

  • To review the application of DNA in protein modification.
  • To highlight DNA's roles in enhancing efficiency and selectivity.
  • To explore DNA as a template, guidance system, and catalyst.

Main Methods:

  • Review of literature on DNA-mediated protein modification.
  • Analysis of DNA's templating function for reactant concentration.
  • Examination of DNA's guidance capabilities for protein binding and selectivity.
  • Assessment of DNA's catalytic potential in modification reactions.

Main Results:

  • DNA can concentrate dilute reactants, increasing reaction efficiency.
  • DNA acts as a specific guidance system, improving site selectivity.
  • DNA can function as a catalyst or part of a catalytic construct.
  • DNA-mediated approaches offer enhanced accuracy in protein modification.

Conclusions:

  • DNA is a versatile tool for advancing protein modification techniques.
  • DNA-based strategies offer significant improvements in efficiency and selectivity.
  • These methods hold promise for future applications in proteomics and therapeutics.