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Generation and Culturing of Primary Human Keratinocytes from Adult Skin
Published on: December 22, 2017
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Keratinocyte differentiation and proteolytic pathways in skin (patho) physiology
Eleni Zingkou1, Georgios Pampalakis2, Georgia Sotiropoulou1
1Department of Pharmacy, School of Health Sciences, University of Patras, Rion-Patras, Greece.
The International Journal of Developmental Biology
|December 9, 2021
Summary
Abnormal skin cell differentiation and protein breakdown are key to Netherton syndrome, seborrheic dermatitis, and psoriasis. These processes are interconnected, driving the pathology of these common skin diseases.
Area of Science:
- Dermatology
- Molecular Biology
- Biochemistry
Background:
- The epidermis acts as a crucial barrier, primarily composed of keratinocytes undergoing differentiation.
- The stratum corneum, the outermost epidermal layer, consists of anucleated corneocytes.
Purpose of the Study:
- To investigate the roles of epidermal differentiation and proteolysis in skin diseases.
- To analyze skin biopsies from Netherton syndrome (NS) models, patients with NS, seborrheic dermatitis (SD), psoriasis, and healthy controls.
Main Methods:
- Histology and immunohistochemistry were employed to analyze skin biopsies.
- Expression levels of key proteins like involucrin, loricrin, and desmosomal proteins were assessed.
Main Results:
- NS, SD, and psoriasis exhibit abnormal epidermal differentiation (hyperplasia, hyperkeratosis, parakeratosis).
- Increased epidermal proteolysis and kallikrein-related peptidases (KLKs) expression were noted in NS and psoriasis.
- Altered expression of desmosomal proteins was observed, linking proteolysis and differentiation.
Conclusions:
- Abnormal epidermal differentiation and proteolysis are interconnected processes.
- These interconnected processes are fundamental to the pathology of NS, SD, and psoriasis.
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