Increasing the Image Contrast via Fast Fluorescence Photobleaching

This paper focuses on the analysis of image sequences acquired during fast photobleaching using a standard wide-field microscope. We show that the photobleaching rate estimated for each pixel is not constant for the whole field of view, but it provides a new spatially variant parametric image related to the cell structure and diffusion of fluorophores. We also provide an alternative way to estimate a pixel-wise photobleaching rate with significantly less computation time than exponential model fitting.Clinical Relevance- This method provides an additional way how fluorescence photobleaching might be used for increasing the image contrast.