Related Experiment Video
Updated: Oct 10, 2025

Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses
Published on: June 13, 2018
[Dolosigranulum pigrum in corneal abscess]
Karina L Roitman1, Marisa N Almuzara2, Mario Javier Fernández Rodriguez1
1Instituto Nacional de Enfermedades Infecciosas (INEI)-Administración Nacional de Laboratorios e Institutos de Salud (ANLIS) Dr. Carlos G. Malbrán, Ciudad Autónoma de Buenos Aires, Argentina.
Abstract:
Dolosigranulum pigrum is a gram-positive, facultatively anaerobic coccus, which is part of the oral and upper respiratory tract microbiota. Although reports of infections by this microorganism are scarce, it has been associated with a wide spectrum of infectious diseases. The case of an elderly man with a lower corneal abscess, in which Dolosigranulum pigrum was isolated, is described. The microorganism was identified by mass spectrometry (MALDI-TOF MS) and by the sequencing of the 16S rRNA gene. Furthermore, the presumptive identification of the causative agent was achieved by using key phenotypic tests such as the cluster arrangement in Gram stain, the negative catalase test, the production of pyrrolidonyl arylamidase and leucine aminopeptidase activity, the growth in 6.5% NaCl and esculin hydrolysis. The data from the literature (and the present case) support the association of the microorganism with ocular infections, which often take a destructive course, mainly in elderly patients.
Insights
Dolosigranulum pigrum, a common bacterium, can cause severe corneal abscesses, particularly in elderly individuals. This case highlights the importance of identifying this often-overlooked pathogen in serious eye infections.
Area of Science:
- Microbiology
- Ophthalmology
- Infectious Diseases
Background:
- Dolosigranulum pigrum is a gram-positive coccus found in the oral and respiratory tracts.
- While typically commensal, it has been linked to various infections, though rarely reported.
Observation:
- A case study involving an elderly male patient with a lower corneal abscess is presented.
- Dolosigranulum pigrum was successfully isolated from the patient's corneal abscess.
Findings:
- Microorganism identification was confirmed using MALDI-TOF MS and 16S rRNA gene sequencing.
- Phenotypic tests including Gram stain, catalase, enzyme activities, salt tolerance, and esculin hydrolysis aided presumptive identification.
Implications:
- This case, along with literature data, supports Dolosigranulum pigrum's association with ocular infections.
- Such infections can be destructive, especially in elderly patients, necessitating prompt diagnosis and treatment.
Related Concept Videos
Angle Closure Glaucoma: Treatment
Glaucoma: Overview

