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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
Published on: February 24, 2015
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Longitudinal DNA methylation dynamics as a practical indicator in clinical epigenetics.
Shohei Komaki1, Hideki Ohmomo1, Tsuyoshi Hachiya1
1Division of Biomedical Information Analysis, Iwate Tohoku Medical Megabank Organization, Disaster Reconstruction Center, Iwate Medical University, 1-1-1, Idaidori, Yahaba, Iwate, 028-3694, Japan.
Clinical Epigenetics
|December 14, 2021
Summary
DNA methylation is generally stable over three months, but dynamic sites link to immune traits. Stable sites are linked to metabolism, offering insights for clinical epigenetics.
Area of Science:
- Epigenetics
- Genomics
- Clinical Medicine
Background:
- DNA methylation is a key epigenetic mechanism.
- Its longitudinal dynamics under normal conditions are poorly understood.
- This study investigates short-term DNA methylation changes.
Purpose of the Study:
- To characterize longitudinal DNA methylation dynamics.
- To differentiate between stable and dynamic CpGs (cytosine methylation).
- To explore the functional implications of methylation stability.
Main Methods:
- Analysis of high-frequency longitudinal DNA methylation data.
- Utilized data from two Japanese males with 24 time-points over three months.
- Employed the Illumina HumanMethylation450 BeadChip probe set.
Main Results:
- Most CpGs remained stable over the three-month period.
- Dynamic CpGs were associated with immune and inflammatory traits.
- Stable CpGs were enriched in metabolism-related genes and less likely to be EWAS markers.
Conclusions:
- CpG methylation stability varies and is functionally relevant.
- Stable and dynamic CpGs can serve as indicators in clinical epigenetics.
- Understanding these dynamics aids in outlining epigenetic mechanisms.
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