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Long Non-Coding RNA Small Nucleolar RNA Host Gene 17 Promotes Hepatocellular Carcinoma Progression by Inhibiting p57
Xu Peng1, Rui Zou1, Caiyan Pan1
1Department of Hepatobiliary and Pancreatic Surgery, Hainan Cancer Hospital, Haikou, Hainan, China.
Objective:
Long non-coding RNA (lncRNA) small nucleolar RNA host gene 17 (lncRNA SNHG17) is dysregulated in many types of human tumors and acts as a tumor suppressor or promoter according to the tumor type. However, the role of lncRNA SNHG17 in hepatocellular carcinoma (HCC) has been uninvestigated.
Materials:
Counting on qRT-PCR assay, lncRNA SNHG17 expression in HCC tissues and cell lines (PLC, Hep3B, Huh7 and 7721) was investigated. By utilizing CCK-8 and flow cytometry assays, cell viability and cell cycle were evaluated. qRT-PCR and western blot was implemented to examine p15, p16, p21, p27 and p57 expression levels in HCC cells.
Results:
We firstly found that expression levels of lncRNA SNHG17 in HCC tissues and cell lines was higher than that in para-carcinoma tissues and QSG-7701 cells (P<0.05). In addition, we observed that patients with tumor size ≥ 5cm, HCC stages III-IV or poor pathological histological types, lncRNA SNHG17 expression were significantly higher than those with tumor size <5 cm, HCC stages I-II or good histopathological types (P<0.05). In vitro studies showcased that low expression of lncRNA SNHG17 restrained HCC cell proliferation and evoked cell cycle arrest. Further, silence of lncRNA SNHG17 up-regulated p57 expression in HCC cells.
Conclusions:
Our study shows that lncRNA SNHG17 plays a role as a tumor promoter in HCC, which may provide new clues for the treatment of HCC.
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