Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[TIPS dysfunction: what has to be done?]

Angiologiia i sosudistaia khirurgiia = Angiology and vascular surgery·2026

Same author

[Early results of transjugular intrahepatic portosystemic shunting in patients with complications of portal hypertension].

Angiologiia i sosudistaia khirurgiia = Angiology and vascular surgery·2026

Same author

[Thromboaspiration for acute upper limb ischemia in a patient with COVID-19 (case report)].

Angiologiia i sosudistaia khirurgiia = Angiology and vascular surgery·2026

Same author

Whole-Genome Sequencing Uncovers Metabolic and Immune System Variations in Propionibacterium freudenreichii Isolates.

Acta naturae·2026

Same author

Comprehensive Characterization of Five Lactococcus Strains: From Phenotypic Traits to Genomic Features.

Acta naturae·2026

Same author

[Successful repeated endovascular procedure for TIPS thrombosis].

Khirurgiia·2025

Search research articles

Related Experiment Video

Updated: Oct 9, 2025

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

Loop-Mediated Isothermal Amplification as a Promising Method for Mass COVID-19 Diagnostics.

J A Makarova¹, K A Fomicheva², A I Osipyants^2,3

¹Faculty of Biology and Biotechnology, Higher School of Economics National Research University, 101000 Moscow, Russia.

Applied Biochemistry and Microbiology

|December 20, 2021

Summary

Loop-mediated isothermal amplification (LAMP) offers a faster and simpler alternative to real-time reverse-transcription polymerase chain reaction (RT-PCR) for early COVID-19 detection. This method holds significant potential for widespread adoption in diagnostics.

Keywords:

COVID-19 LAMP SARS-CoV-2 coronavirus diagnostics loop-mediated isothermal amplification

More Related Videos

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

Related Experiment Videos

Last Updated: Oct 9, 2025

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

Area of Science:

Biotechnology
Molecular Diagnostics
Infectious Disease Research

Background:

Real-time reverse-transcription polymerase chain reaction (RT-PCR) is the standard for early COVID-19 diagnosis.
RT-PCR requires specialized equipment and trained personnel, limiting its accessibility.
Loop-mediated isothermal amplification (LAMP) presents a promising alternative due to its speed and simplicity.

Purpose of the Study:

To highlight the advantages of LAMP over RT-PCR for COVID-19 diagnostics.
To discuss the technical and methodological considerations for the widespread adoption of LAMP.
To explore the potential of LAMP for mass application in COVID-19 testing.

Main Methods:

Comparative analysis of diagnostic methodologies (RT-PCR vs. LAMP).
Discussion of technical requirements and operational simplicity of LAMP.
Review of existing LAMP-based diagnostic kits and their availability.

Main Results:

LAMP is faster and simpler than RT-PCR for COVID-19 detection.
LAMP does not necessitate expensive laboratory equipment or highly trained staff.
While LAMP kits exist, their widespread implementation is still limited.

Conclusions:

LAMP technology has significant potential for broad application in COVID-19 diagnostics.
Addressing technical and methodological aspects is crucial for scaling up LAMP adoption.
LAMP offers a viable solution for accessible and rapid infectious disease testing.